Mapping Short Affinity Tags on Bacterial S-layer with Antibody

Jilin Tang, Andreas Ebner, Bernhard Kraxberger, Helga Badelt-Lichtblau, Hermann Gruber, U.B. Sleytr, N. Ilk, Peter Hinterdorfer

Research output: Contribution to journal › Article › peer-review

Abstract

In conclusion, the interaction between the Strep-tagII and anti-Strep-tagII was explored at the single molecular level. Strep-tagII in the two-dimensional crystalline S-layer protein lattices on a silicon surface could be detected by anti-Strep-tagII antibodies coupled to AFM tip using single-molecule force spectroscopy. The unbinding force between Strep-tagII peptide and anti-Strep-tagII antibody was 27 pN at a loading rate of 177 pNs1.
Original languageEnglish
Pages (from-to)2323-2326
Number of pages4
JournalChemPhysChem
Volume11
DOIs
Publication statusPublished - 2011

Fields of science

  • 103036 Theoretical physics
  • 211904 Biomechanics
  • 103020 Surface physics
  • 210 Nanotechnology
  • 104010 Macromolecular chemistry
  • 106006 Biophysics
  • 106022 Microbiology
  • 106048 Animal physiology
  • 209 Industrial Biotechnology
  • 304 Medical Biotechnology
  • 404 Agricultural Biotechnology, Food Biotechnology
  • 106049 Ultrastructure research
  • 103021 Optics
  • 106002 Biochemistry
  • 104017 Physical chemistry
  • 208 Environmental Biotechnology
  • 104014 Surface chemistry
  • 106023 Molecular biology
  • 107 Other Natural Sciences
  • 301110 Physiology
  • 301206 Pharmacology
  • 206 Medical Engineering
  • 301306 Medical molecular biology
  • 302044 Medical physics
  • 301902 Immunology
  • 305910 Traffic medicine

JKU Focus areas

  • Engineering and Natural Sciences (in general)

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