Functional AFM Imaging of Cellular Membranes Using Functionalized Tips

Lilia Chtcheglova; Peter Hinterdorfer

doi:10.1007/978-1-62703-137-0_20

Functional AFM Imaging of Cellular Membranes Using Functionalized Tips

Lilia Chtcheglova (Editor)
, Peter Hinterdorfer

Department of Applied Experimental Biophysics

Research output: Contribution to journal › Article › peer-review

Abstract

The real-time visualization of specific binding sites on biological samples with high spatial resolution, in order of several nanometers, is an important undertaking in many fields of biology. During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful tool to quickly obtain local receptor nanomaps on complex heterogeneous biosurfaces, such as cells and membranes. In this chapter, we present the TREC technique and explain how to unravel the nano-landscape of cells of the immune system, such as macrophages. We describe the procedures for all steps of the experiment including tip functionalization with Fc fragments via flexible PEG-linker, sample preparation, and localization of Fcγ receptors on macrophages.

Original language	English
Pages (from-to)	359-371
Number of pages	13
Journal	Methods in Molecular Biology
Volume	950
DOIs	https://doi.org/10.1007/978-1-62703-137-0_20
Publication status	Published - 2013

Fields of science

103036 Theoretical physics
211904 Biomechanics
103020 Surface physics
210 Nanotechnology
104010 Macromolecular chemistry
106006 Biophysics
106022 Microbiology
106048 Animal physiology
209 Industrial Biotechnology
304 Medical Biotechnology
404 Agricultural Biotechnology, Food Biotechnology
106049 Ultrastructure research
103021 Optics
106002 Biochemistry
104017 Physical chemistry
208 Environmental Biotechnology
104014 Surface chemistry
106023 Molecular biology
107 Other Natural Sciences
301110 Physiology
301206 Pharmacology
206 Medical Engineering
301306 Medical molecular biology
302044 Medical physics
301902 Immunology
305910 Traffic medicine

JKU Focus areas

Engineering and Natural Sciences (in general)

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10.1007/978-1-62703-137-0_20

Cite this

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abstract = "The real-time visualization of specific binding sites on biological samples with high spatial resolution, in order of several nanometers, is an important undertaking in many fields of biology. During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful tool to quickly obtain local receptor nanomaps on complex heterogeneous biosurfaces, such as cells and membranes. In this chapter, we present the TREC technique and explain how to unravel the nano-landscape of cells of the immune system, such as macrophages. We describe the procedures for all steps of the experiment including tip functionalization with Fc fragments via flexible PEG-linker, sample preparation, and localization of Fcγ receptors on macrophages.",

author = "Lilia Chtcheglova and Peter Hinterdorfer",

year = "2013",

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AB - The real-time visualization of specific binding sites on biological samples with high spatial resolution, in order of several nanometers, is an important undertaking in many fields of biology. During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful tool to quickly obtain local receptor nanomaps on complex heterogeneous biosurfaces, such as cells and membranes. In this chapter, we present the TREC technique and explain how to unravel the nano-landscape of cells of the immune system, such as macrophages. We describe the procedures for all steps of the experiment including tip functionalization with Fc fragments via flexible PEG-linker, sample preparation, and localization of Fcγ receptors on macrophages.

UR - https://www.scopus.com/pages/publications/84870533627

U2 - 10.1007/978-1-62703-137-0_20

DO - 10.1007/978-1-62703-137-0_20

M3 - Article

SN - 1940-6029

VL - 950

SP - 359

EP - 371

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Functional AFM Imaging of Cellular Membranes Using Functionalized Tips

Abstract

Fields of science

JKU Focus areas

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