Force spectroscopy of the fibrin(ogen) – fibrinogen interaction

Lilia Chtcheglova; Andre Haeberli; Giovanni Dietler

doi:10.1002/bip.20910

Force spectroscopy of the fibrin(ogen) – fibrinogen interaction

Lilia Chtcheglova, Andre Haeberli, Giovanni Dietler

Department of Applied Experimental Biophysics

Research output: Contribution to journal › Article › peer-review

Abstract

Fibrin aggregation is of vital importance in many physiological and pathological processes, such as blood coagulation, wound healing, and thrombosis. In the present study, we investigated the forces involved in the initial steps of the fibrinogen fibrin aggregation by force spectroscopy using the atomic force microscope. Our data confirm the existence of strong specific interactions between fibrin and fibrin(ogen), with unbinding forces ranging from 290 to 375 pN and a logarithmic dependence on the loading rate between 0.8 and 23 nN/s.

Original language	English
Pages (from-to)	292-301
Number of pages	10
Journal	Biopolymers
Volume	89
Issue number	4
DOIs	https://doi.org/10.1002/bip.20910
Publication status	Published - 2008

Fields of science

210006 Nanotechnology
103 Physics, Astronomy
106006 Biophysics
106023 Molecular biology
301114 Cell biology

JKU Focus areas

Nano-, Bio- and Polymer-Systems: From Structure to Function
Engineering and Natural Sciences (in general)

Access to Document

10.1002/bip.20910

Cite this

@article{bd972a0f26984592bc7cf094d45eb58b,

title = "Force spectroscopy of the fibrin(ogen) – fibrinogen interaction",

abstract = "Fibrin aggregation is of vital importance in many physiological and pathological processes, such as blood coagulation, wound healing, and thrombosis. In the present study, we investigated the forces involved in the initial steps of the fibrinogen fibrin aggregation by force spectroscopy using the atomic force microscope. Our data confirm the existence of strong specific interactions between fibrin and fibrin(ogen), with unbinding forces ranging from 290 to 375 pN and a logarithmic dependence on the loading rate between 0.8 and 23 nN/s.",

author = "Lilia Chtcheglova and Andre Haeberli and Giovanni Dietler",

year = "2008",

doi = "10.1002/bip.20910",

language = "English",

volume = "89",

pages = "292--301",

journal = "Biopolymers",

issn = "1097-0282",

publisher = "Wiley",

number = "4",

}

TY - JOUR

T1 - Force spectroscopy of the fibrin(ogen) – fibrinogen interaction

AU - Chtcheglova, Lilia

AU - Haeberli, Andre

AU - Dietler, Giovanni

PY - 2008

Y1 - 2008

N2 - Fibrin aggregation is of vital importance in many physiological and pathological processes, such as blood coagulation, wound healing, and thrombosis. In the present study, we investigated the forces involved in the initial steps of the fibrinogen fibrin aggregation by force spectroscopy using the atomic force microscope. Our data confirm the existence of strong specific interactions between fibrin and fibrin(ogen), with unbinding forces ranging from 290 to 375 pN and a logarithmic dependence on the loading rate between 0.8 and 23 nN/s.

AB - Fibrin aggregation is of vital importance in many physiological and pathological processes, such as blood coagulation, wound healing, and thrombosis. In the present study, we investigated the forces involved in the initial steps of the fibrinogen fibrin aggregation by force spectroscopy using the atomic force microscope. Our data confirm the existence of strong specific interactions between fibrin and fibrin(ogen), with unbinding forces ranging from 290 to 375 pN and a logarithmic dependence on the loading rate between 0.8 and 23 nN/s.

U2 - 10.1002/bip.20910

DO - 10.1002/bip.20910

M3 - Article

SN - 1097-0282

VL - 89

SP - 292

EP - 301

JO - Biopolymers

JF - Biopolymers

IS - 4

ER -