Determination of Withanolides and Withanosides in Ashwagandha Based Products Using HPLC-Drift-Tube-Ion-Mobility Quadrupole Time-of-Flight Mass Spectrometry

Laura Zellner; Markus Wierer; Markus Himmelsbach; Benjamin Schatzmann; Bernhard Thalhamer; Christian Klampfl

doi:10.1002/elps.202400188

Determination of Withanolides and Withanosides in Ashwagandha Based Products Using HPLC-Drift-Tube-Ion-Mobility Quadrupole Time-of-Flight Mass Spectrometry

Laura Zellner
, Markus Wierer
, Markus Himmelsbach
, Benjamin Schatzmann
, Bernhard Thalhamer
, Christian Klampfl^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

A total of 19 products based on extracts from Withania somnifera (L.) Dunal, better known by its more common name ashwagandha, and five products based on ashwagandha root powder were analyzed with respect to their content in the biologically relevant substances belonging to the group of withanolides and withanosides. Using HPLC coupled to drift-tube ion-mobility quadrupole time-of-flight mass spectrometry (DT-IM-QTOF-MS), 19 withanolides and withanosides could be tentatively identified. The comparison of the results from the quantitative analysis with the information on the product labels showed that the percentage of withanolides and withanosides deviated from the stated specifications by at least a factor of two and at most a factor of 35.

Original language	English
Pages (from-to)	340-346
Number of pages	7
Journal	Electrophoresis
Volume	46
Issue number	5-6
DOIs	https://doi.org/10.1002/elps.202400188
Publication status	Published - 18 Feb 2025

Fields of science

104002 Analytical chemistry
104 Chemistry
104001 General chemistry

JKU Focus areas

Sustainable Development: Responsible Technologies and Management

Access to Document

10.1002/elps.202400188

Cite this

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title = "Determination of Withanolides and Withanosides in Ashwagandha Based Products Using HPLC-Drift-Tube-Ion-Mobility Quadrupole Time-of-Flight Mass Spectrometry",

abstract = "A total of 19 products based on extracts from Withania somnifera (L.) Dunal, better known by its more common name ashwagandha, and five products based on ashwagandha root powder were analyzed with respect to their content in the biologically relevant substances belonging to the group of withanolides and withanosides. Using HPLC coupled to drift-tube ion-mobility quadrupole time-of-flight mass spectrometry (DT-IM-QTOF-MS), 19 withanolides and withanosides could be tentatively identified. The comparison of the results from the quantitative analysis with the information on the product labels showed that the percentage of withanolides and withanosides deviated from the stated specifications by at least a factor of two and at most a factor of 35.",

author = "Laura Zellner and Markus Wierer and Markus Himmelsbach and Benjamin Schatzmann and Bernhard Thalhamer and Christian Klampfl",

year = "2025",

month = feb,

day = "18",

doi = "10.1002/elps.202400188",

language = "English",

volume = "46",

pages = "340--346",

journal = "Electrophoresis",

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number = "5-6",

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TY - JOUR

T1 - Determination of Withanolides and Withanosides in Ashwagandha Based Products Using HPLC-Drift-Tube-Ion-Mobility Quadrupole Time-of-Flight Mass Spectrometry

AU - Zellner, Laura

AU - Wierer, Markus

AU - Himmelsbach, Markus

AU - Schatzmann, Benjamin

AU - Thalhamer, Bernhard

AU - Klampfl, Christian

PY - 2025/2/18

Y1 - 2025/2/18

N2 - A total of 19 products based on extracts from Withania somnifera (L.) Dunal, better known by its more common name ashwagandha, and five products based on ashwagandha root powder were analyzed with respect to their content in the biologically relevant substances belonging to the group of withanolides and withanosides. Using HPLC coupled to drift-tube ion-mobility quadrupole time-of-flight mass spectrometry (DT-IM-QTOF-MS), 19 withanolides and withanosides could be tentatively identified. The comparison of the results from the quantitative analysis with the information on the product labels showed that the percentage of withanolides and withanosides deviated from the stated specifications by at least a factor of two and at most a factor of 35.

AB - A total of 19 products based on extracts from Withania somnifera (L.) Dunal, better known by its more common name ashwagandha, and five products based on ashwagandha root powder were analyzed with respect to their content in the biologically relevant substances belonging to the group of withanolides and withanosides. Using HPLC coupled to drift-tube ion-mobility quadrupole time-of-flight mass spectrometry (DT-IM-QTOF-MS), 19 withanolides and withanosides could be tentatively identified. The comparison of the results from the quantitative analysis with the information on the product labels showed that the percentage of withanolides and withanosides deviated from the stated specifications by at least a factor of two and at most a factor of 35.

UR - https://www.scopus.com/pages/publications/85219700869

U2 - 10.1002/elps.202400188

DO - 10.1002/elps.202400188

M3 - Article

C2 - 39965076

SN - 1522-2683

VL - 46

SP - 340

EP - 346

JO - Electrophoresis

JF - Electrophoresis

IS - 5-6

ER -

Determination of Withanolides and Withanosides in Ashwagandha Based Products Using HPLC-Drift-Tube-Ion-Mobility Quadrupole Time-of-Flight Mass Spectrometry

Abstract

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