Combined Single Molecule Recognition Imaging and Force Spectroscopy to Study the Interactions Between Uncoupling Proteins and Purine Nucleotides

Melanie Koehler; Gabriel Pürstinger; Rong Zhu; Anne Rupprecht; Hermann Gruber; E. E. Pohl; Peter Hinterdorfer

doi:10.1016/j.bpj.2013.11.1306

Combined Single Molecule Recognition Imaging and Force Spectroscopy to Study the Interactions Between Uncoupling Proteins and Purine Nucleotides

Melanie Koehler
, Gabriel Pürstinger
, Rong Zhu
, Anne Rupprecht
, Hermann Gruber
, E. E. Pohl
, Peter Hinterdorfer

Department of Applied Experimental Biophysics

Research output: Contribution to journal › Article › peer-review

Abstract

We combined recognition imaging and force spectroscopy so as to study the interactions between receptors and ligands on the single molecule level. This method allowed the selection of a single receptor molecule reconstituted in a supported lipid membrane at low density, with the subsequent quantification of the receptor-ligand unbinding force. Based on atomic force microscopy (AFM) tapping mode a cantilever tip carrying a ligand molecule was oscillated across a membrane. Topography and recognition Images of reconstituted receptors were recorded simultaneously by analysing the downwards and upwards parts of the oscillation, respectively.

Original language	English
Pages (from-to)	223a
Number of pages	1
Journal	Biophysical Journal
Volume	106
Issue number	2
DOIs	https://doi.org/10.1016/j.bpj.2013.11.1306
Publication status	Published - Jan 2014

Fields of science

103 Physics, Astronomy
106006 Biophysics

JKU Focus areas

Engineering and Natural Sciences (in general)

Access to Document

10.1016/j.bpj.2013.11.1306

Cite this

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title = "Combined Single Molecule Recognition Imaging and Force Spectroscopy to Study the Interactions Between Uncoupling Proteins and Purine Nucleotides",

abstract = "We combined recognition imaging and force spectroscopy so as to study the interactions between receptors and ligands on the single molecule level. This method allowed the selection of a single receptor molecule reconstituted in a supported lipid membrane at low density, with the subsequent quantification of the receptor-ligand unbinding force. Based on atomic force microscopy (AFM) tapping mode a cantilever tip carrying a ligand molecule was oscillated across a membrane. Topography and recognition Images of reconstituted receptors were recorded simultaneously by analysing the downwards and upwards parts of the oscillation, respectively.",

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AU - Koehler, Melanie

AU - Pürstinger, Gabriel

AU - Zhu, Rong

AU - Rupprecht, Anne

AU - Gruber, Hermann

AU - Pohl, E. E.

AU - Hinterdorfer, Peter

PY - 2014/1

Y1 - 2014/1

N2 - We combined recognition imaging and force spectroscopy so as to study the interactions between receptors and ligands on the single molecule level. This method allowed the selection of a single receptor molecule reconstituted in a supported lipid membrane at low density, with the subsequent quantification of the receptor-ligand unbinding force. Based on atomic force microscopy (AFM) tapping mode a cantilever tip carrying a ligand molecule was oscillated across a membrane. Topography and recognition Images of reconstituted receptors were recorded simultaneously by analysing the downwards and upwards parts of the oscillation, respectively.

AB - We combined recognition imaging and force spectroscopy so as to study the interactions between receptors and ligands on the single molecule level. This method allowed the selection of a single receptor molecule reconstituted in a supported lipid membrane at low density, with the subsequent quantification of the receptor-ligand unbinding force. Based on atomic force microscopy (AFM) tapping mode a cantilever tip carrying a ligand molecule was oscillated across a membrane. Topography and recognition Images of reconstituted receptors were recorded simultaneously by analysing the downwards and upwards parts of the oscillation, respectively.

U2 - 10.1016/j.bpj.2013.11.1306

DO - 10.1016/j.bpj.2013.11.1306

M3 - Article

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