Calculation of specificity constants Γ for RUBISCO of different species from microcalorimetric data

Joachim Frank; Frank Müh

doi:10.1007/s11120-025-01187-w

Calculation of specificity constants Γ for RUBISCO of different species from microcalorimetric data

Joachim Frank
, Frank Müh^*

^*Corresponding author for this work

Department of Theoretical Biophysics

Research output: Contribution to journal › Article › peer-review

Abstract

Based on earlier data concerning the reaction enthalpy Δ_rH of Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from spinach (Frank et al. Phys Chem Chem Phys 2:1301–1304, 2000), it is shown that the specificity constant Γ, indicating the ability of RUBISCO to discriminate between CO₂ and O₂ as substrate, can be determined from an isothermal titration calorimetric (ITC) measurement of Δ_rH as a function of the substrate concentration ratio ρ = [CO₂]/[O₂]. The approach does not need any radioactive materials and might be a cost-effective alternative for RUBISCO engineering to screen for variants with a high specificity constant.

Original language	English
Article number	63
Pages (from-to)	1 - 6
Number of pages	6
Journal	Photosynthesis Research
Volume	163
Issue number	6
DOIs	https://doi.org/10.1007/s11120-025-01187-w
Publication status	Published - 01 Dec 2025

Fields of science

103 Physics, Astronomy

JKU Focus areas

Sustainable Development: Responsible Technologies and Management

Access to Document

10.1007/s11120-025-01187-wLicence: CC BY

Cite this

@article{1f74e019615b411b874fa3c5c89999b4,

title = "Calculation of specificity constants Γ for RUBISCO of different species from microcalorimetric data",

abstract = "Based on earlier data concerning the reaction enthalpy ΔrH of Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from spinach (Frank et al. Phys Chem Chem Phys 2:1301–1304, 2000), it is shown that the specificity constant Γ, indicating the ability of RUBISCO to discriminate between CO2 and O2 as substrate, can be determined from an isothermal titration calorimetric (ITC) measurement of ΔrH as a function of the substrate concentration ratio ρ = [CO2]/[O2]. The approach does not need any radioactive materials and might be a cost-effective alternative for RUBISCO engineering to screen for variants with a high specificity constant.",

author = "Joachim Frank and Frank M{\"u}h",

year = "2025",

month = dec,

day = "1",

doi = "10.1007/s11120-025-01187-w",

language = "English",

volume = "163",

pages = "1 -- 6",

journal = "Photosynthesis Research",

issn = "0166-8595",

publisher = "Springer",

number = "6",

}

TY - JOUR

T1 - Calculation of specificity constants Γ for RUBISCO of different species from microcalorimetric data

AU - Frank, Joachim

AU - Müh, Frank

PY - 2025/12/1

Y1 - 2025/12/1

N2 - Based on earlier data concerning the reaction enthalpy ΔrH of Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from spinach (Frank et al. Phys Chem Chem Phys 2:1301–1304, 2000), it is shown that the specificity constant Γ, indicating the ability of RUBISCO to discriminate between CO2 and O2 as substrate, can be determined from an isothermal titration calorimetric (ITC) measurement of ΔrH as a function of the substrate concentration ratio ρ = [CO2]/[O2]. The approach does not need any radioactive materials and might be a cost-effective alternative for RUBISCO engineering to screen for variants with a high specificity constant.

AB - Based on earlier data concerning the reaction enthalpy ΔrH of Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from spinach (Frank et al. Phys Chem Chem Phys 2:1301–1304, 2000), it is shown that the specificity constant Γ, indicating the ability of RUBISCO to discriminate between CO2 and O2 as substrate, can be determined from an isothermal titration calorimetric (ITC) measurement of ΔrH as a function of the substrate concentration ratio ρ = [CO2]/[O2]. The approach does not need any radioactive materials and might be a cost-effective alternative for RUBISCO engineering to screen for variants with a high specificity constant.

UR - https://www.scopus.com/pages/publications/105023452307

U2 - 10.1007/s11120-025-01187-w

DO - 10.1007/s11120-025-01187-w

M3 - Article

C2 - 41324710

SN - 0166-8595

VL - 163

SP - 1

EP - 6

JO - Photosynthesis Research

JF - Photosynthesis Research

IS - 6

M1 - 63

ER -

Calculation of specificity constants Γ for RUBISCO of different species from microcalorimetric data

Abstract

Fields of science

JKU Focus areas

Access to Document

Other files and links

Cite this