STIM1 protein studied by high resolution solid and solution state NMR

Description

STIM1 is a calcium sensor protein in the endoplasmic reticulum which extends into the cytosol and forms oligomers upon calcium store depletion. [1] The cytosolic part of STIM1 consists of one long (CC1) and two short coiled coil domains involved in homo-oligomerization and spatial elongation of the STIM1 protein leading to acti- vation of the Orai/CRAC channel. [2] The Stormorken syndrome disease associated with a single point mutation (R304W) within this region of STIM1 results in per- manent activation of Orai channel. [3] Using high resolution solid and solution state NMR we have found a helix elongation within the CC1 domain of the mutant close to the mutation position with respect to the wild type STIM1. [4] These findings corroborate the increased propensity of CC1 to form homomers destabilizing the resting state of STIM1, which leads to the channel activation for the Stormorken mutant.

Period	28 Mar 2018
Event title	BISSL (Bilateral Impromptu Symposium (Stockholm-Linz) on NMR Spectroscopy
Event type	Conference
Location	AustriaShow on map