Parallel DNA Amplification Using Localized Microwave Heating in Standard Microtubes

Description

This work presents the utilization of an open-ended semirigid coaxial cable as a miniaturized microwave heating source used in standard 0.2ml microtubes for PCR amplification of single DNA-molecules. With our set-up we achieved the successful amplification of single molecules in a microscopic format. Specifically, single molecules are compartmentalized in microscopic reaction volumes formed by an emulsion. The amplification product is captured on microscopic paramagnetic beads that can then be analyzed under a microscope. Using this small PCR format, we have proof of principle that our device can be used in miniature PCR and is suitable for lab-on-a-chip developments. Moreover, the ease of fabrication and the utilization of standard power RF-components make this set-up suitable for mobile developments also in other technologies.

Period	05 Oct 2010
Event title	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS)
Event type	Conference
Location	NetherlandsShow on map