Fs-Laser-Induced Micro- and Nanostructures on Polycarbonate and Cellulose Acetate Butyrate for Cell Alignment

Laser-generated structures have a huge potential to induce an alignment of
biological cells, which may be important for various fields in medicine and biotechnology.
We describe the formation of fs-laser-induced micro- and nanostructures for achieving the
directed growth of Schwann cells, a type of glial cell that can support the regeneration of
nerve pathways by guiding the neuronal axons in the direction of their alignment. Polymer
surfaces, i.e., polycarbonate (PC) or cellulose acetate butyrate (CAB), were exposed to
the beam of a 1040 nm Yb-based amplified fs-laser system with a pulse length of about
350 fs. With appropriate parameters, the laser exposure resulted in a surface topography
with oriented micro-grooves, which, for PC, were covered with nano-ripples. Schwann
cell growth on these substrates was inspected after 3 to 5 days of cultivation by means of
scanning electron microscopy (SEM). We show that Schwann cells can grow in a certain
direction, predetermined by micro-groove or nano-ripple orientation. In contrast, cells cul-
tivated on randomly oriented nanofibers or unstructured surfaces show an omnidirectional
growth behavior. This method may be used in the future to produce nerve conduits for the
treatment of injuries to the peripheral nervous system