Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

Ted S Acott; Michael P Fautsch; Weiming Mao; C Ross Ethier; Alex S Huang; Mary J Kelley; Mini Aga; Sanjoy K Bhattacharya; Terete Borras; Diane Bovenkamp; Uttio Roy Chowdhury; Abbot F Clark; Mohammed I Dibas; Yiqin Du; Michael H Elliott; Jennifer A Faralli; Haiyan Gong; Samuel Herberg; Murray A Johnstone; Paul L Kaufman; Kate E Keller; Ruth A Kelly; David Krizaj; Markus H Kuehn; Hoi Lam Li; Raquel Lieberman; Shan C Lin; Yutao Liu; Fiona S McDonnell; Colleen M McDowell; Gillian J McLellan; Philip Mzyk; Kayarat Saidas Nair; Darryl R Overby; Donna M Peters; VijayKrishna Raghunathan; Ponugoti Vasantha Rao; Gavin W Roddy; Najam A Sharif; Myoung Sup Shim; Yang Sun; Benjamin R Thomson; Carol B Toris; Colin E Willoughby; Hao F Zhang; Thomas F Freddo; Rudolf Fuchshofer; Kamisha R Hill; Alireza Karimi; Krishnakumar Kizhatil; Casey C Kopcyznski; Paloma Liton; Gaurang Patel; Michael Peng; Padmanabhan P Pattabiraman; Ganesh Prasanna; Ester Reina-Torres; E Griffen Samples; John R Samples; Cynthia L Steel; Clemens A Strohmaier; Preeti Subramanian; Chenna Kesavulu Sugali; Joseph van Batenburg-Sherwood; Cydney Wong; Hannah Youngblood; Gulab S Zode; Elizabeth White; W Daniel Stamer

doi:10.1167/iovs.65.14.32

Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

Ted S Acott^*
, Michael P Fautsch
, Weiming Mao
, C Ross Ethier
, Alex S Huang
, Mary J Kelley
, Mini Aga
, Sanjoy K Bhattacharya
, Terete Borras
, Diane Bovenkamp
, Uttio Roy Chowdhury
, Abbot F Clark
, Mohammed I Dibas
, Yiqin Du
, Michael H Elliott
, Jennifer A Faralli
, Haiyan Gong
, Samuel Herberg
, Murray A Johnstone
, Paul L Kaufman

Kate E Keller, Ruth A Kelly, David Krizaj, Markus H Kuehn, Hoi Lam Li, Raquel Lieberman, Shan C Lin, Yutao Liu, Fiona S McDonnell, Colleen M McDowell, Gillian J McLellan, Philip Mzyk, Kayarat Saidas Nair, Darryl R Overby, Donna M Peters, VijayKrishna Raghunathan, Ponugoti Vasantha Rao, Gavin W Roddy, Najam A Sharif, Myoung Sup Shim, Yang Sun, Benjamin R Thomson, Carol B Toris, Colin E Willoughby, Hao F Zhang, Thomas F Freddo, Rudolf Fuchshofer, Kamisha R Hill, Alireza Karimi, Krishnakumar Kizhatil, Casey C Kopcyznski, Paloma Liton, Gaurang Patel, Michael Peng, Padmanabhan P Pattabiraman, Ganesh Prasanna, Ester Reina-Torres, E Griffen Samples, John R Samples, Cynthia L Steel, Clemens A Strohmaier, Preeti Subramanian, Chenna Kesavulu Sugali, Joseph van Batenburg-Sherwood, Cydney Wong, Hannah Youngblood, Gulab S Zode, Elizabeth White, W Daniel Stamer

^*Korrespondierende/r Autor/-in für diese Arbeit

Universitätsklinik für Augenheilkunde und Optometrie

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

Abstract

Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.

Originalsprache	Englisch
Aufsatznummer	32
Seitenumfang	21
Fachzeitschrift	Investigative Ophthalmology & Visual Science
Volume	65
Ausgabenummer	14
DOIs	https://doi.org/10.1167/iovs.65.14.32
Publikationsstatus	Veröffentlicht - 02 Dez. 2024

Wissenschaftszweige

302007 Augenheilkunde

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10.1167/iovs.65.14.32

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Acott, T. S., Fautsch, M. P., Mao, W., Ethier, C. R., Huang, A. S., Kelley, M. J., Aga, M., Bhattacharya, S. K., Borras, T., Bovenkamp, D., Chowdhury, U. R., Clark, A. F., Dibas, M. I., Du, Y., Elliott, M. H., Faralli, J. A., Gong, H., Herberg, S., Johnstone, M. A., ... Stamer, W. D. (2024). Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches. Investigative Ophthalmology & Visual Science, 65(14), Artikel 32. https://doi.org/10.1167/iovs.65.14.32

@article{b01c22fb57aa4b30a4162733f6cd8bbf,

title = "Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches",

abstract = "Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.",

keywords = "Animals, Humans, Anterior Eye Segment, Aqueous Humor/physiology, Consensus, Glaucoma/physiopathology, Intraocular Pressure/physiology, Organ Culture Techniques, Perfusion/methods, Trabecular Meshwork",

author = "Acott, \{Ted S\} and Fautsch, \{Michael P\} and Weiming Mao and Ethier, \{C Ross\} and Huang, \{Alex S\} and Kelley, \{Mary J\} and Mini Aga and Bhattacharya, \{Sanjoy K\} and Terete Borras and Diane Bovenkamp and Chowdhury, \{Uttio Roy\} and Clark, \{Abbot F\} and Dibas, \{Mohammed I\} and Yiqin Du and Elliott, \{Michael H\} and Faralli, \{Jennifer A\} and Haiyan Gong and Samuel Herberg and Johnstone, \{Murray A\} and Kaufman, \{Paul L\} and Keller, \{Kate E\} and Kelly, \{Ruth A\} and David Krizaj and Kuehn, \{Markus H\} and Li, \{Hoi Lam\} and Raquel Lieberman and Lin, \{Shan C\} and Yutao Liu and McDonnell, \{Fiona S\} and McDowell, \{Colleen M\} and McLellan, \{Gillian J\} and Philip Mzyk and Nair, \{Kayarat Saidas\} and Overby, \{Darryl R\} and Peters, \{Donna M\} and VijayKrishna Raghunathan and Rao, \{Ponugoti Vasantha\} and Roddy, \{Gavin W\} and Sharif, \{Najam A\} and Shim, \{Myoung Sup\} and Yang Sun and Thomson, \{Benjamin R\} and Toris, \{Carol B\} and Willoughby, \{Colin E\} and Zhang, \{Hao F\} and Freddo, \{Thomas F\} and Rudolf Fuchshofer and Hill, \{Kamisha R\} and Alireza Karimi and Krishnakumar Kizhatil and Kopcyznski, \{Casey C\} and Paloma Liton and Gaurang Patel and Michael Peng and Pattabiraman, \{Padmanabhan P\} and Ganesh Prasanna and Ester Reina-Torres and Samples, \{E Griffen\} and Samples, \{John R\} and Steel, \{Cynthia L\} and Strohmaier, \{Clemens A\} and Preeti Subramanian and Sugali, \{Chenna Kesavulu\} and \{van Batenburg-Sherwood\}, Joseph and Cydney Wong and Hannah Youngblood and Zode, \{Gulab S\} and Elizabeth White and Stamer, \{W Daniel\}",

year = "2024",

month = dec,

day = "2",

doi = "10.1167/iovs.65.14.32",

language = "English",

volume = "65",

journal = "Investigative Ophthalmology \& Visual Science",

issn = "0146-0404",

publisher = "Association for Research in Vision and Ophthalmology (ARVO)",

number = "14",

}

Acott, TS, Fautsch, MP, Mao, W, Ethier, CR, Huang, AS, Kelley, MJ, Aga, M, Bhattacharya, SK, Borras, T, Bovenkamp, D, Chowdhury, UR, Clark, AF, Dibas, MI, Du, Y, Elliott, MH, Faralli, JA, Gong, H, Herberg, S, Johnstone, MA, Kaufman, PL, Keller, KE, Kelly, RA, Krizaj, D, Kuehn, MH, Li, HL, Lieberman, R, Lin, SC, Liu, Y, McDonnell, FS, McDowell, CM, McLellan, GJ, Mzyk, P, Nair, KS, Overby, DR, Peters, DM, Raghunathan, V, Rao, PV, Roddy, GW, Sharif, NA, Shim, MS, Sun, Y, Thomson, BR, Toris, CB, Willoughby, CE, Zhang, HF, Freddo, TF, Fuchshofer, R, Hill, KR, Karimi, A, Kizhatil, K, Kopcyznski, CC, Liton, P, Patel, G, Peng, M, Pattabiraman, PP, Prasanna, G, Reina-Torres, E, Samples, EG, Samples, JR, Steel, CL, Strohmaier, CA, Subramanian, P, Sugali, CK, van Batenburg-Sherwood, J, Wong, C, Youngblood, H, Zode, GS, White, E & Stamer, WD 2024, 'Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches', Investigative Ophthalmology & Visual Science, Jg. 65, Nr. 14, 32. https://doi.org/10.1167/iovs.65.14.32

TY - JOUR

T1 - Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

AU - Acott, Ted S

AU - Fautsch, Michael P

AU - Mao, Weiming

AU - Ethier, C Ross

AU - Huang, Alex S

AU - Kelley, Mary J

AU - Aga, Mini

AU - Bhattacharya, Sanjoy K

AU - Borras, Terete

AU - Bovenkamp, Diane

AU - Chowdhury, Uttio Roy

AU - Clark, Abbot F

AU - Dibas, Mohammed I

AU - Du, Yiqin

AU - Elliott, Michael H

AU - Faralli, Jennifer A

AU - Gong, Haiyan

AU - Herberg, Samuel

AU - Johnstone, Murray A

AU - Kaufman, Paul L

AU - Keller, Kate E

AU - Kelly, Ruth A

AU - Krizaj, David

AU - Kuehn, Markus H

AU - Li, Hoi Lam

AU - Lieberman, Raquel

AU - Lin, Shan C

AU - Liu, Yutao

AU - McDonnell, Fiona S

AU - McDowell, Colleen M

AU - McLellan, Gillian J

AU - Mzyk, Philip

AU - Nair, Kayarat Saidas

AU - Overby, Darryl R

AU - Peters, Donna M

AU - Raghunathan, VijayKrishna

AU - Rao, Ponugoti Vasantha

AU - Roddy, Gavin W

AU - Sharif, Najam A

AU - Shim, Myoung Sup

AU - Sun, Yang

AU - Thomson, Benjamin R

AU - Toris, Carol B

AU - Willoughby, Colin E

AU - Zhang, Hao F

AU - Freddo, Thomas F

AU - Fuchshofer, Rudolf

AU - Hill, Kamisha R

AU - Karimi, Alireza

AU - Kizhatil, Krishnakumar

AU - Kopcyznski, Casey C

AU - Liton, Paloma

AU - Patel, Gaurang

AU - Peng, Michael

AU - Pattabiraman, Padmanabhan P

AU - Prasanna, Ganesh

AU - Reina-Torres, Ester

AU - Samples, E Griffen

AU - Samples, John R

AU - Steel, Cynthia L

AU - Strohmaier, Clemens A

AU - Subramanian, Preeti

AU - Sugali, Chenna Kesavulu

AU - van Batenburg-Sherwood, Joseph

AU - Wong, Cydney

AU - Youngblood, Hannah

AU - Zode, Gulab S

AU - White, Elizabeth

AU - Stamer, W Daniel

PY - 2024/12/2

Y1 - 2024/12/2

N2 - Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.

AB - Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.

KW - Animals

KW - Humans

KW - Anterior Eye Segment

KW - Aqueous Humor/physiology

KW - Consensus

KW - Glaucoma/physiopathology

KW - Intraocular Pressure/physiology

KW - Organ Culture Techniques

KW - Perfusion/methods

KW - Trabecular Meshwork

UR - https://www.scopus.com/pages/publications/85212880418

U2 - 10.1167/iovs.65.14.32

DO - 10.1167/iovs.65.14.32

M3 - Article

C2 - 39693082

SN - 0146-0404

VL - 65

JO - Investigative Ophthalmology & Visual Science

JF - Investigative Ophthalmology & Visual Science

IS - 14

M1 - 32

ER -