Initial NMR and other spectroscopic studies of PsbO protein from Photosystem II

Beschreibung

PsbO, also called 33 kDa thylakoid protein, is the biggest extrinsic protein of PS II located on lumenal surface of this photosynthetic apparatus. Currently a Cryo-EM structure with resolution of 3.2 Å from spinach including PsbO is available. The protein with actual molecular weight 26 kDa has a characteristic �-barrel structure and high content of random coils similar to its cyanobacterial homologue. By in- teracting with other two smaller extrinsic proteins, namely PsbP and PsbQ, the heterotrimeric triangular PsbO–PsbP–PsbQ complex is formed and it serves to op- timize the efficiency of oxygen evolution in PSII under physiological conditions. Content of secondary structure of PsbO was estimated by CD and FTIR and state of the sample prior to NMR measurements was controlled by Raman and fluo- rescence spectroscopy. Currently PsbO has been subjected to NMR experiments yielding incomplete information. In order to overcome line-broadening effect in NMR experiments, expression of deuterated sample was tested. Amino acid selec- tive unlabeling yielded valuable information for identification of peaks and future assignment of PsbO.

Zeitraum	28 März 2018
Ereignistitel	BISSL (Bilateral Impromptu Symposium (Stockholm-Linz) on NMR Spectroscopy
Veranstaltungstyp	Konferenz
Ort	ÖsterreichAuf Karte anzeigen